Human IgE (non-immune)(azide-free, low endotoxin)

Cat.No. DIA HE1

Cat. No.
DIA HE1
Product name
Human IgE (non-immune)(azide-free, low endotoxin)
Description

Human Monoclonal Antibody

Specificity

This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.Must be handled as potentially infectious as all human material.The purity of the human IgE is > 90%, tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.

Immunogen

Non-immune

Subclass

IgE/k

Epitope

Non-immune

Content

100 µl, 1mg/mL

Purification
Protein L purified. Endotoxin level < 25 EU/mg.
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.15 M NaCl
Storage
For long term storage, -18 to -22ºC is recommended, but it may be stored for short term use at 2 to 8ºC. The expiry date refers to storage at -18 to -22ºC. Repeated freeze/thawing is not recommended
Application 1

ELISA
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. 

Application 2

IHC
This antibody can be used in immunochemistry

Application 3

Cell Stimulation Asssay
This antibody can be used in cellular immunology research.

Application 4

Western Blot
This antibody can be used in Western Blotting.

Specific references
Stimulation of human airway smooth muscle (HASM) cell’s with IgE
 
Jyoti Balhara (2012) Role of IgE in modulating the expression and function of smMLCK in human airway smooth muscle cells. A thesis submitted to the Faculty of Graduate Studies of The University of Manitoba.

Redhu NS, Shan L, Al-Subait D, Ashdown HL, Movassagh H, Lamkhioued B,Gounni AS (2013) IgE induces proliferation in human airway smooth muscle cells: role of MAPK and STAT3 pathways. Allergy, Asthma & Clinical Immunology. 9:41

Stimulation of mast cells (MC) with IgE
 
Nunomura S, Shimada S, Kametani Y, Yamada Y, Yoshioka M, Suemizu H, Ozawa M, Itoh T, Kono A, Suzuki R, Tani K, Ando K, Yagita H, Ra C, Habu S, Satake M, Sasaki E (2012) Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset). International Immunology 24:593–603.
 
IgE used as a positive control in microarray technology
 
Nahtman T, Jernberg A, Mahdavifar A, Zerweck J, Schutkowski M, Maeurer M, Reilly M (2007) Validation of peptide epitope microarray experiments and extraction of quality data. J Immunol Methods 328:1-13.
 
Other applications
 
Papamichael KI, Kreuzer MP, Guilbault GG (2007) Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means. Sensor Actuat B-Chem 121:178-186.
Stimulation of human bronchial/tracheal smooth muscle (B/TSM) cells with IgE
1. Redhu NS, Saleh A, Shan L, Gerthoffer WT, Kung SK, Halayko AJ, Lamkhioued B, Gounni AS (2009) Proinflammatory and Th2 Cytokines Regulate the High Affinity IgE Receptor (FceRI) and IgE-Dependant Activation of Human Airway Smooth Muscle Cells. PLoS ONE 4:e6153.
 Stimulation of acute myelogenous leukemia (AML) blasts
2. Bruserud R;, Gjertsen BT, Ulvestad E (2002) Expression of Fc(epsilon)-receptors by human acute myelogenous leukemia (AML) blasts:studies of high- and low- (CD23) affinity receptor expression and the effects of IgE-mediated receptor ligation on functional AML blast characteristics. Leukemia Res 26:515-21.
Stimulation of neutrophils
3. Saffar AS, Alphonse MP, Shan L, HayGlass KT, Simons FER, Gounni AS (2007) IgE modulates neutrophil survival in asthma: Role of mitochondrial pathway. J immunol 178:2535-2541.

Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA)
4. Yamaki K and Yoshino S (2011) Aspergillus oryzae Lectin Induces Anaphylactoid Oedema and Mast Cell Activation Through its Interaction With Fucose of Mast Cell–Bound Non-specific IgE. Scandinavian Journal of Immunology 74:445–453.

Used as a control for IgE in skin graft on mice
5. Fairley JA, Burnett CT, Fu CL, Larson DL, Fleming MG, Giudice GJ (2007) A pathogenic role for IgE in autoimmunity: Bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice. J Invest Dermatol 127:2605-2611.