Anti-NGAL (rat)

Cat.No. ABS 039-15

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Cat. No.
ABS 039-15
Product name
Anti-NGAL (rat)
Description

Mouse monoclonal antibody

Specificity

ABS 039-15 binds rat NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant rat NGAL precursor with C-terminal His-tag coupled to carrier

Application
Species reactivity (positive)
Rat
Species reactivity (negative)
Human, pig, dog, mouse
Subclass

IgG1/k

Gene ID
170496
Clone number
15
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Application 1

ELISA

ABS 039-15 binds free rat NGAL in solution. ABS 039-15 (as capture antibody) forms a sandwich ELISA pair with ABS 039-32 (as biotinylated detection antibody) for measuring rat NGAL (measuring range 4-400 pg/mL in an optimized assay).
 
The figure illustrates the calibration curve of a sandwich assay with ABS 039-15 as the capture antibody and ABS 039-32B as the biotinylated detection antibody.

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A