Anti-NGAL (mouse)

Cat.No. ABS 043-10

Cat. No.
ABS 043-10
Product name
Anti-NGAL (mouse)
Description

Rat monoclonal antibody

Specificity

ABS 043-10 binds NGAL (neutrophil gelatinase-associated lipocalin) in mouse serum.

Immunogen

Recombinant mouse NGAL 

Application
Species reactivity (positive)
Mouse
Species reactivity (negative)
Human, pig, dog, rat
Subclass

IgG1/k

Gene ID
16819
Clone number
10
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mouse neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, 24p3, SIP24, uterocalin and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40 and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated in the liver. but like its mammalian homologues it is upregulated and secreted by the kidney in response to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland.

Application 1
ELISA
ABS 43-10 binds free mouse NGAL in solution. 
ABS 043-10 forms an antibody pair for sandwich ELISA with ABS 043-29. It is recommended to use ABS 043-10 as capture antibody and biotinylated ABS 043-29 as detection antibody.
 
The figure illustrates the calibration curve of a sandwich assay with ABS 043-10 as the capture antibody and ABS 043-29B as the biotinylated detection antibody.
Application 2

WB

ABS 043-10 can be used in Western blotting

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A