Anti-NGAL (dog)

Cat.No. ABS 047-14

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Cat. No.
ABS 047-14
Product name
Anti-NGAL (dog)
Description

Mouse monoclonal antibody

Specificity

ABS 047-14 binds dog NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant dog NGAL 

Application
Species reactivity (positive)
Dog
Species reactivity (negative)
Human, mouse, rat
Subclass

IgG2b/k

Gene ID
491320
Clone number
14
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application 1
ELISA
ABS 047-14 binds free dog NGAL. 
ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay. 
 
The calibration curve of a sandwich assay for Dog NGAL using ABS 047-17 as the capture antibody and ABS 047-14B as the biotinylated detection antibody.
 
Application 2

WB
ABS 047-14 can be used in Western blotting.

Application 3

IHC
ABS 047-14 was used in IHC.

IHC staining in dog kidney tissue. Scattered tubules cells were stained strongly from kidney with acute nephritis. Dilution: 1:50.
Application 4

IHC

ABS 047-17 works in IHC on formalin-fixed, paraffin-embedded sections.