Anti-NGAL (dog)

Cat.No. ABS 047-14B

Cat. No.
ABS 047-14B
Product name
Anti-NGAL (dog)
Description

Mouse monoclonal antibody, biotinylated

Specificity

ABS 047-14 binds dog NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant dog NGAL 

Application
Species reactivity (positive)
Human, mouse, rat
Species reactivity (negative)
Not determined
Subclass

IgG2b/k

Gene ID
491320
Clone number
14
Epitope

Not determined

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application 1

ELISA

ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay. 
 
The calibration curve of a sandwich assay for Dog NGAL using ABS 047-17 as the capture antibody and ABS 047-14B as the biotinylated detection antibody.