Cat.No. HYB 314-02
Mouse monoclonal antibody
Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ)
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
HYB 314-02 reacts equally strong with both the deamidated peptide (KLQPFPQPELPYPQPQ) and the non-deamidated peptide (KLQPFPQPQLPYPQPQ) when coated directly in an immunoplate (1). HYB 314-02 is also usefull as a detection antibody in combination with a polyclonal anti-Gliadin capture antibody (2, 3).
HYB 314-02 can be used in western blot.
A western blot of Gliadin peptide (HYB 314-02) measured in wheat (lane 1) and barley (lane 2). The SDS gel was run under reducing conditions and HYB 314-02 diluted 1:4000.
1. Skovbjerg H, Koch C, Anthonsen D, Sjostrom H (2004) Deamidation and cross-linking of gliadin peptides by transglutaminases and the relation to celiac disease. Biochim Biophys Acta 1690:220-230.
2. Gujral N, Löbenberg R, Surech M, Sunwoo H (2012) In-Vitro and In-Vivo Binding Activity of Chicken Egg Yolk Immunoglobulin Y (IgY) against Gliadin in Food Matrix. J Agric. Food Chem 60:3166-3172.
3. Gujral N, Surech MR, Sunwoo HH (2012) Quantitative Double antibody sandwich ELISA for the determination of gliadin. J. Immunoassay Immunochem. 33:339-351.