Anti-yENaC (y-subunit epithelial sodium channel)

Cat.No. CAM 005-02

Cat. No.
CAM 005-02
Product name
Anti-yENaC (y-subunit epithelial sodium channel)
Description

Mouse monoclonal antibody

Specificity

CAM 005-02 is specific for the inhibitory tract of human yENaC subunit.

Immunogen

The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Clone number
5c2
Epitope

The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 003-07.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the g-subunit and putative

release of a 43-amino acid inhibitory tract from the g-subunit ectodomain.

Application 1

ELISA
A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).

 
The calibration curve of a sandwich assay for Anti-yENaC using CAM 003-07 as the capture antibody and CAM 005-02B as the biotinylated detection antibody. 
Application 2

WB
CAM 005-02 was used in Western Blot.

Application 3

IHC
CAM 005-02 was used in immunohistochemistry.

Application 4

IF
CAM 005-02 was used in immunofluorescence.

1. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).