Cat.No. CAM 005-02
Mouse monoclonal antibody
CAM 005-02 is specific for the inhibitory tract of human yENaC subunit.
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to amino acid residue 139-160 of human yENaC subunit.
The epitope is on the inhibitory tract of human yENaC. The epitope differs from that of CAM 003-07.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
The epithelial sodium channel (ENaC) of the kidney is necessary for extracellular volume homeostasis and normal arterial BP. Activity of ENaC is enhanced by proteolytic cleavage of the g-subunit and putative
release of a 43-amino acid inhibitory tract from the g-subunit ectodomain.
A sandwich ELISA can be made using CAM 003-07 (1-4 μg/ml) as the capture antibody and biotinylated CAM 005-02 (0.05 - 0.2 μg/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
|The calibration curve of a sandwich assay for Anti-yENaC using CAM 003-07 as the capture antibody and CAM 005-02B as the biotinylated detection antibody.|
CAM 005-02 was used in Western Blot.
CAM 005-02 was used in immunohistochemistry.
CAM 005-02 was used in immunofluorescence.
1. Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The Epithelial Sodium Channel g-Subunit Is Processed Proteolytically in Human Kidney. J Am Soc Nephrol. 2014 Jul 24. pii: ASN.2013111173. (epub ahead of print).