Anti-Pregnancy associated plasma protein A (human, PAPP-A)

Cat.No. ABS 006-01

Cat. No.
ABS 006-01
Product name
Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Description

Mouse monoclonal antibody

Specificity

ABS 006-01 is specific for native human PAPP-A

Immunogen

Purified human PAPP-A

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
5069
Clone number
PAPP A 1-1
Epitope

The epitope of ABS 006-01 is different from BTE 004-09 and ABS 001-24. The epitope is located on the PAPP-A subunit of the heterotetramer.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).

Application 1

ELISA
In sandwich ELISA with a polyclonal antibody in coat, ABS 006-01 only reacts poorly with PAPP-A in third trimester serum. 

Application 2

WB
ABS 006-01 only detects unreduced PAPP-A in Western blotting. 

Application 3
IHC
ABS 006-01 can be used in IHC. Please consult www.proteinatlas.org
Paraffin-embedded human placenta tissue immunostained with ABS 006-01 (www.proteinatlas.org)
Immunohistochemical staining of a formalin-fixed, paraffin-embedded placental tissue with ABS 006-01