Anti-Pregnancy associated plasma protein A (human, PAPP-A)

Cat.No. ABS 006-24

Cat. No.
ABS 006-24
Product name
Anti-Pregnancy associated plasma protein A (human, PAPP-A)
Description

Mouse monoclonal antibody

Specificity

ABS 006-24 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A .

Immunogen

Purified human PAPP-A

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
5069
Clone number
PAPP A 1-24
Epitope

The epitope of ABS 006-24 is different from BTE 004-09 and ABS 006-01. The epitope is located on the PAPP-A subunit of the heterotetramer.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).

Application 1

ELISA
BTE 004-09 as coating antibody and biotinylated ABS 006-24 as detection antibody is a good combination for sandwich ELISA.

Application 2

WB
ABS 006-24 only detecs unreduced PAPP-A in Western Blotting.

Application 3

IHC
ABS 006-24 gives the strongest singal in IHC compared to ABS 006-01 and BTE 004-09.
 

Immunohistochemical staining of a formalin-fixed, paraffin-embedded placental tissue with ABS 006-24