Anti-NGAL (rat)

Cat.No. ABS 039-08

Cat. No.
ABS 039-08
Product name
Anti-NGAL (rat)
Description

Mouse monoclonal antibody

Specificity

ABS 039-08 binds rat NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant rat NGAL precursor 

Application
Species reactivity (positive)
Rat
Species reactivity (negative)
Human, pig, dog, mouse
Subclass

IgG1/k

Gene ID
170496
Clone number
8
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Application 1

ELISA

ABS 039-08 binds free rat NGAL in solution.
Application 2

IHC

ABS 039-08 reacts with rat NGAL, when used in Immunohistochemistry. (1)
 
NGAL immunostaining in kidney, Formalin-fixed, paraffin-embedded tissue sections of cortical epithelial cells in the S1/S2 segments (proximal convoluted tubules) following DXR-induced acute kidney injury. Top panel, saline treatment; bottom panel, DXR treatment; X630 (1).

1. Zhang J, Ohm J,Vaidya V, Espandiari P, Knapton A,  Goering P, Brown R, Bonventre J, Herman E (2009) NGAL Expression in Kidney of Spontaneously Hypertensive Rats (SHR) Treated with Doxorubicin (DXR) Alone or Combined with Dexrazoxane (DRZ) (Presented at Tox Expo 2009)