Anti-NGAL (pig)

Cat.No. ABS 048-28

Cat. No.
ABS 048-28
Product name
Anti-NGAL (pig)
Description

Mouse monoclonal antibody

Specificity

ABS 048-28 binds pig NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant pig NGAL 

Application
Species reactivity (positive)
Pig
Species reactivity (negative)
Human, dog, mouse, rat
Subclass

IgG1/k

Gene ID
100153501
Clone number
28
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Pig neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Application 1
ELISA
ABS 048-28 binds free pig NGAL in solution. 
ABS 048-28 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 048-17 (as capture antibody) for measuring porcine NGAL.
 
The calibration curve of a sandwich assay for Pig NGAL using ABS 048-17 as the capture antibody and ABS 048-28B as the biotinylated detection antibody.
Application 2

WB

ABS 048-28 can be used in Western blotting.
Application 3

IHC

ABS 048-28 detects NGAL in paraffin-embedded sections in the cytoplasm of renal tubular cell from pig.
 
NGAL expression in the ischemic injured pig kidney; detected with anti-pig NGAL antibody (ABS 048-28; antibody dilution 1:25), using the Ventana Immunostainer BENCHMARK XT from Roche. Image and data supplied by Dr. Despoina Karandrea, Aretaieion Hospotal, Athens, Greece.

 

Positive staining of renal tubular cell cytoplasm, using mouse monoclonal antibody against pig NGAL (Cat. no. ABS 048-28), dilution 1:1000. Courtesy of Oscar McCook, University of Ulm.

 

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A