Anti-NGAL (monkey)

Cat.No. ABS 063-56

Cat. No.
ABS 063-56
Product name
Anti-NGAL (monkey)
Description

Mouse monoclonal antibody

Specificity

ABS 063-56 reacts with NGAL (neutrophil gelatinase-associated lipocalin) from Macaca mulatta (Rhesus) and Macaca facicularis (Cynomolgus)

Immunogen

Recombinant monkey NGAL

Application
Species reactivity (positive)
Monkey
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
3934
Clone number
56
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Monkey neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Application 1
ELISA
ABS 063-56 binds free monkey NGAL
ABS 063-56 (as capture antibody) forms a sandwich ELISA pair with ABS 063-68 (as biotinylated detection antibody) for measuring monkey NGAL.
 
The calibration curve of a sandwich assay for Monkey NGAL using ABS 063-56 as the capture antibody and ABS 063-68B as the biotinylated detection antibody.
Application 2

IHC

ABS 063-56 reacts with monkey NGAL in IHC paraffin sections.
 
 
IHC staining for nephrotoxicant-induced NGAL protein expression in male Cynomolgus monkey using the BioPorto Diagnostics antibody (ABS 063-56) at 1:50. Mild multifocal staining was localized in apical surface of intact tubular epithelial cells in the cortex and medulla (left panel) and marked multifocal tubular cast (granular and cellular) in the cortex (right panel). Image: Kindly provided by McDuffie, J.E., J.Y. Ma et al (Janssen Research & Development, L.L.C.)

1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A