Anti-NGAL (human)

Cat.No. ABS 038-14B

Cat. No.
ABS 038-14B
Product name
Anti-NGAL (human)
Description

Mouse monoclonal antibody, biotinylated

Specificity

ABS 038-14 binds human NGAL (neutrophil gelatinase-associated lipocalin)

Immunogen

Recombinant human NGAL 

Application
Species reactivity (positive)
Human, pig, dog
Species reactivity (negative)
Mouse, rat
Subclass

IgG1/k

Gene ID
3934, 100153501, 491320
Clone number
14
Epitope

Differs from those of HYB 211-01, -02, ABS 038-04, -15, -26 and the ABS 062 series of antibodies.

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.

Application 1

ELISA
ABS 038-15 and ABS 038-26 (as biotinylated detection antibodies) for measuring human NGAL. With ABS 038-15B detection antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis. With ABS 038-14B detection antibody, the assay is specific for NGAL homodimer and can be used at sample dilutions giving low levels of total NGAL.

The calibration curve of a sandwich assay for Human Dimer NGAL using ABS 038-14 as the capture antibody and ABS 038-14B as the biotinylated detection antibody.