Anti-NGAL (human)

Cat.No. ABS 038-15

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Cat. No.
ABS 038-15
Product name
Anti-NGAL (human)
Description
Mouse monoclonal antibody
Specificity
ABS 038-15 binds human NGAL (neutrophil gelatinase-associated lipocalin)
Immunogen
Recombinant human NGAL 
Application
Species reactivity (positive)
Human
Species reactivity (negative)
Dog. pig, mouse, rat
Subclass
IgG1/k
Clone number
15
Epitope
Differs from those of HYB 211, ABS 038 and the ABS 062 series of antibodies.
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.
Application 1
ELISA
ABS 038-15 binds human NGAL in solution and coated onto a solid phase. 
ABS 038-15 (as capture antibody) forms sandwich pairs with HYB 211-02, ABS 038-04, ABS 038-14, ABS 038-23 and ABS 038-26 (as biotinylated detection antibodies) for measuring human NGAL. When ABS 038-15 is used as biotinylated detection antibody with ABS 038-14 as capture antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis.
 
The calibration curve of a sandwich assay for Total Human NGAL using ABS 038-14 as the capture antibody and ABS 038-15B as the biotinylated detection antibody. 
Application 2
WB
ABS 038-15 can be used in Western blotting.