Anti-MBL (human)

Cat.No. HYB 131-01

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Cat. No.
HYB 131-01
Product name
Anti-MBL (human)
Description

Mouse monoclonal antibody

Specificity

HYB 131-01 is specific for MBL (mannan-binding lectin) from human serum or plasma.

Immunogen

MBL purified from human donor plasma 

Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
4153
Clone number
3B6
Epitope

The epitope is on the head-neck region of the MBL protein chain. Prior binding of the antibody is thought to block binding to carbohydrate. The epitope differs from that of HYB 131-10 and HYB 131-11. 

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.

Application 1

ELISA
HYB 131-01 is selective for normally oligomerized MBL when used as detection antibody in sandwich ELISA with HYB 131-01 coat (1).
HYB 131-01 can also be used in a manan assay, when coating with manan and using HYB 131-01B as the biotinylated detection antibody (2).

 
The calibration curve of a sandwich assay for MBL using HYB 131-01 as the capture antibody and HYB 131-01B as the biotinylated detection antibody.
 
Application 2

WB
In Western blotting, HYB 131-01 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. A dilution guideline of 1/1000 has proved successful (2).

Application 3

IHC
HYB 131-01 is also well suited for immunohistochemistry on human tissue samples, frozen or paraffin embedded, from (3, 4). Please consult www.proteinatlas.org
 

Paraffin-embedded human liver tissue immunostained with HYB 131-01 (3)

 

 

Paraffin-embedded human cancerous liver tissue immunostained with HYB 131-01 (www.proteinatlas.org)

 

1. Garred P, Madsen HO, Kurtzhals JA, Lamm LU, Thiel S, Hey AS, Svejgaard A (1992) Diallelic polymorphism may explain variations of the blood concentration of mannan-binding protein in Eskimos, but not in black Africans. Eur J Immunogenet 19:403-412.

2. Ramos GP, Nisihara R, Maestri CA, Bichalho MG, Carvalho NS, Reason IM (2013) MBL serum Concentration in women with HPV presenting CIN III lesions. Human Immunology 74:67-69

3.Garred P, Larsen F, Madsen HO,  Koch C (2003) Mannose-binding lectin deficiency - revisited. Mol Immunol 40:73-84.

4. Hornum M, Bay JT, Clausen P, Melchior Hansen J, Mathiesen ER, Feldt-Rasmussen B et al. High levels of mannose-binding lectin are associated with lower pulse wave velocity in uraemic patients. B M C Nephrology. 2014;15:162.

5. Nisihara M, Magrini F, Mocelin V, Messias-Reason IJ (2013) Deposition of the lectin pathway of complement in renal biopsies of lupus nephritis patients. Hum.Immunol. 74: 907-910