Cat.No. HYB 131-01B
Mouse monoclonal antibody, biotinylated
HYB 131-01 is specific for MBL (mannan-binding lectin) from human serum or plasma.
MBL purified from human donor plasma
The epitope is on the head-neck region of the MBL protein chain. Prior binding of the antibody is thought to block binding to carbohydrate. The epitope differs from that of HYB 131-10 and HYB 131-11.
50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars. It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, dependent on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.
|The calibration curve of a sandwich assay for MBL using HYB 131-01 as the capture antibody and HYB 131-01B as the biotinylated detection antibody.|
1. Fiane AE, Videm V, Lingaas PS, Heggelund L, Nielsen EW, Geiran OR, Fung M, Mollnes TE (2003) Mechanism of Complement Activation and Its Role in the Inflammatory Response After Thoracoabdominal Aortic Aneurysm Repair. Circulation 108:849-856.
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