Anti-MBL (human)

Cat.No. HYB 131-10

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Cat. No.
HYB 131-10
Product name
Anti-MBL (human)
Description

Mouse monoclonal antibody

Specificity

HYB 131-10 is specific for MBL (mannan-binding lectin) from human serum or plasma.

Immunogen

MBL purified from human donor plasma.

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
4153
Clone number
1E2
Epitope

The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-11.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consists of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum.

Application 1

ELISA

HYB 131-10 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting HYB 131-10 reacts with human MBL in both its oligomerized state and as single protein chain of 26 kDa. HYB 131-10 can be used to measure MBL in plasma or serum but is not specific for oligomerized MBL when used in combination with itself or other MBL antibodies(1).
Application 2

WB

HYB 131-10 can be used in Western blotting.
1. Hein E, Honoré C, Skjoedt MO, Munthe-Fog L, Hummelshøj T, Garred P. (2010) Functional analysis of
Ficolin-3 mediated complement activation. PlosOne 5(11):e15443. doi: 10.1371/journal.pone.0015443.