Anti-H-Ficolin (human)

Cat.No. RIG 334-01B

Cat. No.
RIG 334-01B
Product name
Anti-H-Ficolin (human)
Description
Mouse monoclonal antibody, biotinylated
Specificity
RIG 334-01 reacts specifically with H-ficolin. No cross-reaction has been observed with L- or M-ficolin.
Immunogen
Recombinant human H-ficolin 
Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
8547
Clone number
FCN334
Epitope
Not determined
Content
100 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region. 
Application 1
ELISA
RIG 334-01 reacts with recombinant H-ficolin coated directly onto the microtiter well. A sandwich ELISA for H-ficolin uses RIG 334-01 for both capture and detection (1).
1. Munthe-Fog L, Hummelshøj T, Ma YJ, Hansen BE, Koch C, Madsen HO, Skjødt K, Garred P (2008) Characterization of a polymorphism in the coding sequence of FCN3 resulting in a Ficolin-3 (Hakata antigen) deficiency state. Molecular Immunology 45:2660-2666.