Anti-Glucagon (human, pig, rat)

Cat.No. ABS 058-08B

Cat. No.
ABS 058-08B
Product name
Anti-Glucagon (human, pig, rat)
Description

Mouse monoclonal antibody, biotinylated

Specificity

ABS 058-08 binds human glucagon.

Immunogen

Synthetic N-terminal fragment of glucagon coupled to carrier

Application
Species reactivity (positive)
Human, pig, rat
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641, 24952, 397595
Clone number
08
Epitope

The epitope lies within the N-terminal half of human glucagon

Content

50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2 in the L cells of the intestinal mucosa.

Application 1

ELISA

ABS 058-08 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 061-09 (as capture antibody) for measuring glucagon.
 
The calibration curve of a sandwich assay for Glucagon using ABS 061-09 as the capture antibody and ABS 058-08B as the biotinylated detection antibody.