Anti-Glucagon (human, pig, rat)

Cat.No. ABS 061-09

Cat. No.
ABS 061-09
Product name
Anti-Glucagon (human, pig, rat)
Description

Mouse monoclonal antibody

Specificity

ABS 061-09 binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.

Immunogen

Synthetic C-terminal fragment of glucagon coupled to carrier

Application
Species reactivity (positive)
Humna, pig, rat
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2641, 24952, 397595
Clone number
09
Epitope

The epitope lies within the C-terminal region of glucagon.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2 in the L cells of the intestinal mucosa.

Application 1
ELISA
ABS 061-09 binds free glucagon in solution. 
ABS 061-09 when used as capture antibody forms a sandwich ELISA pair with ABS 058-08 as biotinylated detection antibody for measuring glucagon.
 
The calibration curve of a sandwich assay for Glucagon using ABS 061-09 as the capture antibody and ABS 058-08B as the biotinylated detection antibody.
Application 2

IHC
ABS 061-09 has been used in IHC.

Formalin fixed paraffin-embedded human pancreas tissue stained with ABS 061-09. Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000
Application 3

IF
ABS 061-09 has been used in IF.

IF staining in human pancreas tissue stained with ABS 061-09. Signal is strong in alpha cells. Dilution: 1:4000.