Anti-Gelsolin (human)

Cat.No. ABS 017-20

Delivery: 1-5 days

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Cat. No.
ABS 017-20
Product name
Anti-Gelsolin (human)
Description

Mouse monoclonal antibody

Specificity

ABS 017-20 binds human gelsolin

Immunogen

Gelsolin purified from human plasma

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2934
Clone number
20
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.

Application 1

ELISA

ABS 017-20 does not work as capture antibody in sandwich ELISA.
Application 2

IF
ABS 017-20 can be used in Immunofluorescence.
Please consult www.proteinatlas.org

 
Immunofluorescent staining of human cell line U-251 MG shows positivity in actin filaments.
Antibody dilution: 1:2000 (www.proteinatlas.org)
Application 3

IHC
ABS 017-20 can be used in Immunohistochemistry.
Please consult www.proteinatlas.org

Immunohistochemical staining of human kidney with ABS 017-20 shows cytoplasmic and membranous positivity in both renal tubules and glomeruli.
Antibody dilution: 1:6000 (www.proteinatlas.org)

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