Anti-Gastric Inhibitory Polypeptide (GIP)

Cat.No. ABS 021-04

Cat. No.
ABS 021-04
Product name
Anti-Gastric Inhibitory Polypeptide (GIP)
Description

Mouse monoclonal antibody

Specificity

ABS 021-04 binds human GIP. ABS 021-04 cross-reacts up to 10% with PACAP and human GLP-2 but <0.1% with glucagon, GLP-1 or VIP.

Immunogen

Synthetic human gastric inhibitory polypeptide 

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
2695
Clone number
4
Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa.
GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).
Application 1

ELISA
With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000. In addition ABS 021-04 binds free GIP in solution

ABS 021-04 (as capture antibody) forms a sandwich ELISA pair with ABS 054-24B (as biotinylated
detection antibody) for measuring GIP. Detection limit obtained with our non-optimized buffer system is up
to 4 ng/mL of GIP (1-42).

Application 2

IHC

ABS 021-04 can be used in IHC. Please consult www.proteinatlas.org
 
Paraffin-embedded human duodenum tissue immunostained with ABS 021-04 (www.proteinatlas.org)
Paraffin-embedded human small intestine tissue immunostained with ABS 021-04 (www.proteinatlas.org)

 

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