Anti-Complement factor D (human)

Cat.No. GAU 008-01B

Cat. No.
GAU 008-01B
Product name
Anti-Complement factor D (human)
Description

Mouse monoclonal antibody, biotinylated

Specificity

GAU 008-01 is specific for complement factor D in human serum and plasma (1).

Immunogen

Human factor D (1)

Application
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
1675
Clone number
I8/1b
Epitope

Epitope specificity differs from that of GAU 010-04 (1).

Content

100 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment. 

Application 1

ELISA

GAU 008-01 is used as a biotinylated detection antibody in sandwich ELISA with a GAU 010-04 capture (1). 
 

The calibration curve of a sandwich assay for Factor D using GAU 010-04 as the capture antibody and GAU 008-01B as the biotinylated detection antibody.

1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.