Anti-Complement component C5b-9 (human)

Cat.No. DIA 011-01

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Cat. No.
DIA 011-01
Product name
Anti-Complement component C5b-9 (human)
Description

Mouse monoclonal antibody

Specificity

DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (1). 

Immunogen

Purified C5b-9 

Species reactivity (positive)
Human, pig, horse, baboon
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
727
Clone number
aE11
Epitope

DIA 011-01 binds to a neoepitope exposed on C9 

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Other information
This product is not for further manufacture.
Application 1

ELISA
DIA 011-01 is well suited for quantifying TCC in ELISA. (2,3)

Application 2

WB
DIA 011-01 is not recommended for Western blotting as the epitope is destroyed during the process (4).

Application 3

IHC
TCC in various tissues by immunohistochemistry (frozen and paraffin sections) (1, 2, 4).

IHC staining of frozen tonsil section. Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10.

 

Application 4

IF
DIA 011-01 can be used in immunofluorescence staining (5).

1. Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6

2. Mollnes TE, Lea T, Harboe M, Tschopp J (1985) Monoclonal antibodies recognizing a neoantigen of poly (C9) detect the human terminal complement complex in tissue and plasma. Scand J Immunol 22:183-195.

3. Palarasah,Y, Nielsen C, Sprogøe U, Christensen ML, Lillevang S, Madsen HO, Bygum A, Koch C, Skjodt K, Skjoed MO (2011) Novel assays to assess the functional capacity of the classical, the alternative and the lectin pathways of the complement system.  Clin Exp Immun 164: 388-395.

4. Mollnes TE (1997) Analysis of in vivo complement activation. Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C: Weir's Handbook of Experimental Immunology. Boston, MA: Blackwell Science, pp. 78.1-78.8.

5. Jansen J, Høgåsen K, Mollnes T (1993) Extensive Complement Activation in Hereditary Porcine Membranoproliferative Glomerulonephritis Type 11 (Porcine Dense Deposit Disease. Am J Path 194: 1356-1365.
 

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