Anti-Complement component C5b-9 (human)

Cat.No. DIA 011-01B

Cat. No.
DIA 011-01B
Product name
Anti-Complement component C5b-9 (human)
Description

Mouse monoclonal antibody, biotinylated

Specificity

DIA 011-01 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (1). 

Immunogen

Purified C5b-9 

Application
Species reactivity (positive)
Human, pig, horse, baboon
Species reactivity (negative)
Not determined
Subclass

IgG2a/k

Gene ID
727
Clone number
aE11
Epitope

DIA 011-01 binds to a neoepitope exposed on C9 

Content

100 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and DIA 011-01 is directed against a neoepitope exposed on C9 when incorporated into the TCC.

Application 1

ELISA
DIA 011-01 can be used in ELISA

1. Mollnes TE, Harboe M (1987) Immunohistochemical detection of the membrane and fluid-phase terminal complement complexes C5b-9(m) and SC5b-9. Consequences for interpretation and terminology. Scand J Immunol 26:381-6

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