Cat.No. HAH 002-01
Mouse monoclonal antibody
HAH 002-01 is specific for butyrylcholinesterase from human serum or plasma.
Butyrylcholinesterase isolated from human plasma
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Butyrylcholinesterase (BChE, EC 220.127.116.11.) is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. Butyrylcholinesterase is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits, each with molecular mass of 90 kDa (1).
HAH 002-01 reacts with BChE in normal human serum in sandwich ELISA, using HAH 002-01 as both capture and detection antibody (1). Serum cholinesterase activity can be measured by enzyme antigen immunoassay (EAIA) in combination with HAH 002-01 as capture antibody (2, 3). HAH 002-01 is also applicable in sophisticated immunomagnetic quantification assays for the detection of nerve agent adducts (4, 5).
|The calibration curve of a sandwich assay for Butyrylcholinesterase using HAH 002-01 as the capture antibody and HAH 002-01B as the biotinylated detection antibody.|
In Western blotting after SDS-PAGE no reaction is seen with either the reduced or unreduced form of BChE. No reaction is seen with acetylcholinesterase from human nervous tissue and erythrocytes.
1. Aoki Y, Helzlsouer K, Strickland P (2014) Arylesterase Phenotype-Specific Positive Association Between Arylesterase Activity and Cholinesterase Specific Activity in Human Serum. Int. J. Environ. Res. Public Health 11:1422-1443.
2. Pan Y, Gao D, Yang W, Cho H, Yang G, Tai HH, Zhan CG (2005) Computational redesign of human butyrylcholinesterase for anticocaine medication. Proc Natl Acad Sci 102:16656-61.
3. Yang W, Pan Y, Zheng F, Cho H, Tai HH, Zhan CG (2009) Free-Energy Perturbation Simulation on Transition States and Redesign of Butyrylcholinesterase. Biophysical Journal 96:1931-1938.
4. Sporty J, Lemire S, Jakubowski E, Renner J, Evans R, Williams R, Schmidt J, van der Schans M, Noort D, Johnson R (2010) Immunomagnetic Separation and Quantification of Butyrylcholinesterase Nerve Agent Adducts in Human Serum. Anal Chem 82, 6593-6600.
5. Knaack J, Zhou Y, Abney C, Prezioso S, Magnuson M, Evans R, Jakubowski E, Hardy K, Johnson R (2012) High-Throughput Immunomagnetic Scavenging Technique for Quantitative Analysis of Live VX Nerve Agent in Water, Hamburger, and Soil Matrixes. Anal Chem 84:10052-10057.