Cat.No. ABS 037-21
Mouse monoclonal antibody
ABS 037-21 binds bovine aprotinin immobilized on a solid phase or free in solution.
Aprotinin from bovine lung
The epitope of ABS 037-21 is different from the epitopes of ABS 037-18 and ABS 037-35
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury.
ABS 037-21 (as capture antibody coated at 2 µg/mL) forms an antibody pair with ABS 037-18 (as biotinylated detection antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 µg/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.
|The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using ABS 037-21 as the capture antibody and ABS 0037-18B as the biotinylated detection antibody.|
ABS 037-21 can be used in Western blotting.