Cat.No. HYB 230-01
Mouse monoclonal antibody
HYB 230-01 is specific for human Antithrombin III (AT III) native form
Antithrombin III isolated from human plasma
Epitope specificity differs from that of HYB 230-04, HYB 228-10 and HYB 228-12.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
HYB 230-01 reacts strongly with native AT III. Strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well. In a sandwich ELISA setup, HYB 230-01B acts as a detection antibody in conbination with ABS 014-22 as capture antibody to measure Thrombin-Antithrombin (TAT) complexes.
|The calibration curve of a sandwich assay for TAT (Thrombin-antithrombin) using ABS 014-22 as capture antibody and HYB 230-01B as the biotinylated detection antibody.|